{"id":970,"date":"2021-04-15T12:25:30","date_gmt":"2021-04-15T12:25:30","guid":{"rendered":"https:\/\/shirishpathare.com\/?page_id=970"},"modified":"2021-04-15T12:25:30","modified_gmt":"2021-04-15T12:25:30","slug":"laser-microscope","status":"publish","type":"page","link":"https:\/\/sites.hbcse.tifr.res.in\/shirish\/index.php\/laser-microscope\/","title":{"rendered":"Laser Microscope"},"content":{"rendered":"\n

Aim: To find the average size of microbes in a water sample using laser microscope.<\/p>\n\n\n\n

Apparatus: Water sample with microbe culture, syringe, laser source (wavelength = 635 nm), scale, retort stand, screen<\/p>\n\n\n\n

Introduction:<\/p>\n\n\n\n

A plastic syringe contains water sample with microbe culture. A water droplet is formed at the open end of the syringe when the piston of the syringe is gently pressed. A Laser beam is focused on the droplet. On the screen, we see a magnified image of the microbes present in the water droplet. Around the magnified image of the microbes, we observe a diffraction pattern. By studying this diffraction pattern, we will find their average size.<\/p>\n\n\n\n

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Schematic representation of the set-up<\/p>\n\n\n\n

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Fig.1 The photograph of the pattern obtained on the screen<\/p>\n\n\n\n

Procedure:<\/p>\n\n\n\n

  1. From the photographs given below, generate the relevant data from the diffraction patterns.<\/li>
  2. Devise your procedure to generate the data with 1% uncertainty. Find the average size of microbes present in the water sample used here.<\/li><\/ol>\n\n\n\n